Journal: Scientific reports

Article Title: Immuno-transcriptomic analysis based on machine learning identifies immunity signature genes of chronic rhinosinusitis with nasal polyps.

doi: 10.1038/s41598-025-02508-8

Figure Lengend Snippet: Fig. 7. Gene expression levels of candidate genes. Gene expression levels of CXCR1 (A), CCL13 (B), PPBP (C), CCR3 (D) and MMP9 (E) in CRSwNP and control samples. Gene expression levels of CXCR1 (F), CCL13 (G), PPBP (H), CCR3 (I) and MMP9 (J) in different inflammatory endotypes of CRSwNP. (*P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant).

Article Snippet: The membranes were closed in 5% skimmed milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: CXCR1 (1:500; Servicebio, GB11625-100), PPBP (1:50; Proteintech, 13313-1-AP), GAPDH (1:3000; Servicebio, GB15004-100).

Techniques: Gene Expression, Control

Journal: Scientific reports

Article Title: Immuno-transcriptomic analysis based on machine learning identifies immunity signature genes of chronic rhinosinusitis with nasal polyps.

doi: 10.1038/s41598-025-02508-8

Figure Lengend Snippet: Fig. 8. Candidate gene protein expression levels in normal mucosal tissues and chronic rhinosinusitis with nasal polyps (CRSwNP) tissues were analysed using IHC staining. Unpaired t-tests were used to determine the significant differences for candidate gene protein expression between the normal mucosal(n = 5) and CRSwNP (n = 5) groups. (A) CXCR1. (B) CCL13. (C) PPBP. (D) CCR3. (E) MMP9. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

Article Snippet: The membranes were closed in 5% skimmed milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: CXCR1 (1:500; Servicebio, GB11625-100), PPBP (1:50; Proteintech, 13313-1-AP), GAPDH (1:3000; Servicebio, GB15004-100).

Techniques: Expressing, Immunohistochemistry

Journal: Scientific reports

Article Title: Immuno-transcriptomic analysis based on machine learning identifies immunity signature genes of chronic rhinosinusitis with nasal polyps.

doi: 10.1038/s41598-025-02508-8

Figure Lengend Snippet: Fig. 9. Effects of CXCR1 and PPBP on IL-8 secretion. (A) HNEpC cells were transfected with siRNA (targeting CXCR1), and CXCR1 expression in transfected cells was detected by RT-qPCR. (B) Detection of CXCR1 protein expression by western blot. (C) Detection of IL-8 in the supernatant of CXCR1 knockdown cells using an ELISA kit. (D) HNEpC cells were transfected with PPBP overexpression plasmid and PPBP expression was detected by RT-qPCR in the transfected cells. (E) PPBP protein expression was detected by western blot. (F) The level of IL-8 in the supernatant of PPBP overexpressing cells was detected using ELISA kit.

Article Snippet: The membranes were closed in 5% skimmed milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: CXCR1 (1:500; Servicebio, GB11625-100), PPBP (1:50; Proteintech, 13313-1-AP), GAPDH (1:3000; Servicebio, GB15004-100).

Techniques: Transfection, Expressing, Quantitative RT-PCR, Western Blot, Knockdown, Enzyme-linked Immunosorbent Assay, Over Expression, Plasmid Preparation